| 產(chǎn)品編號(hào) | bsm-55541R |
| 英文名稱 | Transglutaminase 2 Recombinant Rabbit mAb |
| 中文名稱 | 谷氨酰胺轉(zhuǎn)胺酶2重組兔單抗 |
| 別 名 | G ALPHA h; GNAH; TG(C); TG2; TGase 2; TGase C; TGase H; TGC; TGM2; Transglutaminase 2; Transglutaminase C; Transglutaminase H |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 17E10 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1: 500-1: 2000,IHC-P=1: 100-1: 500,IHC-F=1: 100-1: 500,IF=1: 100-1: 500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78 kDa |
| 檢測(cè)分子量 | 78 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TGM2: 560-615/687 |
| 亞 型 | IgG |
| 純化方法 | Affinity Purification |
| 緩 沖 液 | PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol. |
| 保存條件 | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components. Gene ID: 7052 谷氨酰胺轉(zhuǎn)移酶2（TGM2）是一種鈣離子依賴酶，通過催化賴氨酸與谷氨酰胺殘基之間形成化學(xué)鍵，可以將胞質(zhì)和細(xì)胞外基質(zhì)蛋白進(jìn)行交聯(lián)。這種雙功能酶也有內(nèi)在GTP酶活性，研究表明，轉(zhuǎn)酰氨基酶活性的調(diào)節(jié)有可能通過G蛋白偶聯(lián)受體信號(hào)轉(zhuǎn)導(dǎo)通路進(jìn)行調(diào)節(jié)。在交聯(lián)肽中，TGM2可以幫助調(diào)節(jié)細(xì)胞骨架結(jié)構(gòu)、細(xì)胞遷移、凋亡和細(xì)胞基質(zhì)粘附。另外，該酶在創(chuàng)傷愈合與免疫應(yīng)答中起重要作用。TGM2顯示有體外激酶活性，胰島素樣生長(zhǎng)因子結(jié)合蛋白3（IGFBP-3）為其潛在底物。這種廣泛表達(dá)的蛋白定位于胞質(zhì)與胞核之中，但是從細(xì)胞表面與細(xì)胞外基質(zhì) |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Transglutaminase 2 monoclonal antibody, unconjugated (bsm-55541R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Transglutaminase 2 Monoclonal Antibody, Unconjugated(bsm-55541R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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