| 產(chǎn)品編號 | bsm-61065R |
| 英文名稱 | ATP1B1 Recombinant Rabbit mAb |
| 中文名稱 | 鈉鉀ATP酶蛋白b1重組兔單抗 |
| 別 名 | beta 1 Sodium Potassium ATPase; Adenosinetriphosphatase; AT1B1_HUMAN; ATP 1B; ATP1B; ATP1B1; ATPase Na+/K+transporting beta 1 polypeptide; Atpb 1; ATPBS; beta 1 subunit of Na(+) K(+) ATPase; Na K ATPase beta 1 polypeptide; Na+/K+ATPase beta 1 subunit; Sodium/potassium dependent ATPase beta 1 subunit; Sodium/potassium transporting ATPase beta 1 chain; Sodium/potassium transporting ATPase subunit beta 1; Sodium/potassium-dependent ATPase subunit beta-1; Sodium/potassium-transporting ATPase subunit beta-1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 2B9 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human ATP1B1: 261-303 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]. Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Involved in cell adhesion and establishing epithelial cell polarity. Subunit: Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4. Interacts with MLC1 (PubMed:22328087). Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM. Interacts with KIRREL3. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Tissue Specificity: Found in most tissues. Similarity: Belongs to the X(+)/potassium ATPases subunit beta family. SWISS: P05026 Gene ID: 481 Database links: Entrez Gene: 481 Human Entrez Gene: 11931 Mouse Omim: 182330 Human SwissProt: Q24046 Drosophila melanogaster SwissProt: Q9JM72 Guinea pig SwissProt: P05026 Human SwissProt: P14094 Mouse SwissProt: Q9TT37 Rabbit SwissProt: P05028 Sheep Unigene: 4821 Drosophila melanogaster Unigene: 291196 Human Unigene: 4550 Mouse Unigene: 8925 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATP1B1 Monoclonal Antibody, Unconjugated(bsm-61065R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
