| 產(chǎn)品編號(hào) | bs-18814R |
| 英文名稱 | METTL1 Rabbit pAb |
| 中文名稱 | 甲基化樣蛋白1抗體 |
| 別 名 | C12orf1; D1075 like gene product; FLJ95748; Methyltransferase like 1; Methyltransferase like protein 1; Methyltransferase-like protein 1; Mettl1; TRM8; TRMB_HUMAN; TRMT8; tRNA(guanine N(7) ) methyltransferase; tRNA(guanine(46)-N(7))-methyltransferase; tRNA(guanine-N(7)-)-methyltransferase; tRNA(m7G46) methyltransferase; tRNA(m7G46)-methyltransferase; YDL201w. |
| 研究領(lǐng)域 | 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human METTL1: 1-100/276 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008] Function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4. Similarity: Belongs to the methyltransferase superfamily. TrmB family. SWISS: Q9UBP6 Gene ID: 4234 Database links: Entrez Gene: 4234 Human Entrez Gene: 17299 Mouse GenBank: BC000550 Human Omim: 604466 Human SwissProt: Q9UBP6 Human SwissProt: Q9Z120 Mouse Unigene: 42957 Human Unigene: 251593 Mouse |
| 產(chǎn)品圖片 |
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-METTL1 (bs-18814R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 32 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (METTL1) Polyclonal Antibody, Unconjugated (bs-18814R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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