| 產(chǎn)品編號 | bs-10754R |
| 英文名稱 | DNA Ligase III Rabbit pAb |
| 中文名稱 | DNA連接酶3抗體 |
| 別 名 | DNA Ligase 3; D11Wsu78e; DNA ligase; DNA ligase 3; DNA ligase III; DNLI3_HUMAN; LIG 3; LIG2; LIG3; Ligase II, DNA, ATP dependent; Ligase III DNA ATP dependent; MGC78176; OTTMUSP00000000875; Polydeoxyribonucleotide synthase [ATP] 3; Polydeoxyribonucleotide synthase [ATP]; Polydeoxyribonucleotide synthase; RP23-316C11.6. |
| 研究領(lǐng)域 | 細(xì)胞生物 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 113 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DNA Ligase III: 701-800/1009 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Function: Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Subcellular Location: Nucleus. Tissue Specificity: Testis, thymus, prostate and heart. Similarity: Belongs to the ATP-dependent DNA ligase family. Contains 1 BRCT domain. Contains 1 PARP-type zinc finger. SWISS: P49916 Gene ID: 3980 Database links: Entrez Gene: 3980 Human Entrez Gene: 16882 Mouse Omim: 600940 Human SwissProt: P49916 Human SwissProt: P97386 Mouse Unigene: 100299 Human Unigene: 277136 Mouse Unigene: 230056 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(bs-10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DNA Ligase III Polyclonal Antibody, Unconjugated(bs-10754R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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