產(chǎn)品編號(hào) | bs-18448R |
英文名稱(chēng) | LW1/HSFX1 Rabbit pAb |
中文名稱(chēng) | 熱休克轉(zhuǎn)錄因子家族X(qián)連鎖1抗體 |
別 名 | hCG_27154; heat shock transcription factor family X linked 1; Heat shock transcription factor, X-linked; HSFX1; HSFX2; LW 1; LW1. |
研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
克 隆 號(hào) | |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 45 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human LW1/HSFX1: 181-280/423 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
HSFX1 (heat shock transcription factor family, X linked 1), also known as LW-1, is a 423 amino acid protein that is predominately expressed in testis. Localized to the cytoplasm, HSFX1 is thought to be involved in spermatogenesis and male fertility. Subcellular Location: Nucleus (Potential). Cytoplasm. Tissue Specificity: Testis-specific. Similarity: Belongs to the HSF family. SWISS: Q9UBD0 Gene ID: 100506164 Database links: Entrez Gene: 100506164 Human GenBank: NM_016153 Human SwissProt: Q9UBD0 Human Unigene: 709146 Human |
產(chǎn)品圖片 |
Sample:
Jurkat Cell (Human) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Primary: Anti-LW1/HSFX1 (bs-18448R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated (bs-18448R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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