| 產(chǎn)品編號(hào) | bs-11853R |
| 英文名稱 | HMX2 Rabbit pAb |
| 中文名稱 | 同源盒蛋白H6亞型2抗體 |
| 別 名 | H6 family homeo box 2; H6 family homeobox 2; H6 homeo box 2; H6L; HMX 2; HMX2; HMX2_HUMAN; Homeo box(H6 family) 2; Homeo box protein H6 family member 2; Homeobox(H6 family) 2; Homeobox protein H6 family member 2; Homeobox protein HMX2; Nkx5 2. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMX2: 122-200/273 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The homeobox DNA-binding domain is a 60 amino acid motif that is conserved among many species and functions to bind DNA via a helix-turn-helix structure, thereby playing a role in transcriptional regulation and the control of gene expression. HMX2 (H6 family homeobox 2), also known as H6L or Nkx5-2, is a 273 amino acid nuclear protein that belongs to the HMX homeobox family and contains one homeobox DNA-binding domain. HMX2 functions as a transcription factor that assists in specification of neuronal cell types and is essential for proper development of hypothalamus and inner ear. Hemizygous deletions of the gene encoding HMX2 are thought to lead to vestibular dysfunction, inner ear malformations and congenital sensorineural hearing loss. Function: Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development. Subcellular Location: Nucleus. Similarity: Belongs to the HMX homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: A2RU54 Gene ID: 3167 Database links: Entrez Gene: 3167 Human Entrez Gene: 15372 Mouse Omim: 600647 Human SwissProt: A2RU54 Human SwissProt: P43687 Mouse Unigene: 444756 Human Unigene: 303357 Mouse |
| 產(chǎn)品圖片 |
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-HMX2 antibody (bs-11853R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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