| 產(chǎn)品編號 | bs-9671R |
| 英文名稱 | ASH1L Rabbit pAb |
| 中文名稱 | ASH1蛋白抗體 |
| 別 名 | Absent small and homeotic disks protein 1 homolog; Absent Small or Homeotic Like; ASH 1; ASH 1L; ash1(absent small or homeotic 1); Ash1(absent small or homeotic) like; Absent small and homeotic disks protein 1 homolog; ASH1-like protein; ASH-1L; ASH1L_HUMAN; ASH1L1; huASH1; Lysine N-methyltransferase 2H; Probable histone-lysine N-methyltransferase ASH1L. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 327 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ASH1L: 1951-2100/2969 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ASH1L is a 2,969 amino acid protein encoded by the human gene ASH1L. ASH1L belongs to the histone-lysine methyltransferase family (SET2 subfamily) and contains three AT hook DNA-binding domains, one AWS domain, one BAH domain, one bromodomain, one PHD-type zinc finger, one post-SET domain and one SET domain. It is a widely expressed nuclear protein with highest expression found in brain, heart and kidney. ASH1L is a histone methyltransferase and is believed to methylate 'Lys-4' of Histone H3, which is a specific tag for epigenetic transcriptional activation. Function: Histone methyltransferase. Probably methylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation. Subcellular Location: Nucleus. Cell junction, tight junction. Chromosome (Probable). Note=The relevance of tight junction localization is however unclear. Tissue Specificity: Widely expressed, with highest level in brain, heart and kidney. Similarity: Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 3 A.T hook DNA-binding domains. Contains 1 AWS domain. Contains 1 BAH domain. Contains 1 bromo domain. Contains 1 PHD-type zinc finger. Contains 1 post-SET domain. Contains 1 SET domain. SWISS: Q9NR48 Gene ID: 55870 Database links: Entrez Gene: 55870 Human Omim: 607999 Human SwissProt: Q9NR48 Human Unigene: 491060 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ASH1L) Polyclonal Antibody, Unconjugated (bs-9671R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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