| 產(chǎn)品編號 | bs-6466R |
| 英文名稱 | PRDM1 Rabbit pAb |
| 中文名稱 | PRDM1抗體 |
| 別 名 | PRDM1_HUMAN; PR domain zinc finger protein 1; BLIMP1; EC:2.1.1.-; BLIMP-1; BLIMP1; beta-interferon gene positive regulatory domain I-binding factor; PR domain-containing protein 1; Positive regulatory domain I-binding factor 1(PRDI-BF1; PRDI-binding factor 1); PR/SET domain 1; PRDI-BF1; |
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Specific References (3) | bs-6466R has been referenced in 3 publications.
[IF=3.448] Ma Q et al. Non‐coenzyme role of vitamin B1 in RANKL‐induced osteoclastogenesis and ovariectomy induced osteoporosis. J Cell Biochem. 2020 Feb 26. WB ; mouse.
[IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019. WB ; Mouse.
[IF=2.416] Lu, Yinglin. et al. Ascorbic acid and all-trans retinoic acid promote proliferation of chicken blastoderm cells (cBCs) by mediating DNA demethylation. In Vitro Cell Dev-An. 2022 Mar;:1-11 IF ; Chicken.
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| 研究領域 | 細胞生物 免疫學 信號轉(zhuǎn)導 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 92 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRDM1: 501-600/825 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. Subunit: Interacts with PRMT5. Subcellular Location: Nucleus. Cytoplasm. Similarity: Contains 4 C2H2-type zinc fingers. Contains 1 SET domain. SWISS: O75626 Gene ID: 639 Database links: Entrez Gene: 639 Human Entrez Gene: 12142 Mouse SwissProt: O75626 Human SwissProt: Q60636 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRDM1) Polyclonal Antibody, Unconjugated (bs-6466R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
