| 產(chǎn)品編號 | bs-8202R |
| 英文名稱 | BRN4 Rabbit pAb |
| 中文名稱 | 腦轉錄因子4蛋白抗體 |
| 別 名 | class 3; transcription factor 4; Brain specific homeobox POU domain protein 4; Brain-4; Brain-specific homeobox/POU domain protein 4; BRAIN4; Brn-4; BRN4; DFN3; Oct-9; Octamer-binding protein 9; Octamer-binding transcription factor 9; OTF-9; OTF9; PO3F4_HUMAN; POU domain; POU domain class 3 transcription factor 4; POU3F4. |
| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | Flow-Cyt=1μg /Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BRN4: 211-320/361 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain.Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) . A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. Function: Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain. Subcellular Location: Nucleus. Tissue Specificity: Brain specific. DISEASE: Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) [MIM:304400]. A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. Similarity: Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: P49335 Gene ID: 5456 Database links: Entrez Gene: 5456 Human Entrez Gene: 18994 Mouse Omim: 300039 Human SwissProt: P49335 Human SwissProt: P62515 Mouse Unigene: 2229 Human Unigene: 405149 Mouse Unigene: 56946 Mouse Unigene: 33030 Rat |
| 產(chǎn)品圖片 |
Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody:Rabbit Anti-BRN4 antibody(bs-8202R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
