| 產(chǎn)品編號(hào) | bs-8176R |
| 英文名稱(chēng) | PHF8 Rabbit pAb |
| 中文名稱(chēng) | 組蛋白賴(lài)氨酸去甲基化酶PHF8抗體 |
| 別 名 | PHD finger protein 8; PHD finger protein 8; Histone lysine demethylase PHF8; PHD finger protein 8; PHF8_HUMAN; ZNF422. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 表觀(guān)遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 118 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PHF8: 501-600/1060 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] Function: Histone lysine demethylase with selectivity for thedi-and monomethyl states that plays a key role cell cycleprogression, rDNA transcription and brain development. Demethylatesmono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 andH3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylatedhistone H4 'Lys-20' residue (H4K20Me1). Acts as a transcriptionactivator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigeneticrepressive marks. Involved in cell cycle progression by beingrequired to control G1-S transition. Acts as a coactivator of rDNAtranscription, by activating polymerase I (pol I) mediatedtranscription of rRNA genes. Required for brain development,probably by regulating expression of neuron-specific genes. Onlyhas activity toward H4K20Me1 when nucleosome is used as a substrateand when not histone octamer is used as substrate. May also haveweak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however,the relevance of this result remains unsure in vivo. Specificallybinds trimethylated 'Lys-4' of histone H3 (H3K4me3), affectinghistone demethylase specificity: has weak activity toward H3K9Me2in absence of H3K4me3, while it has high activity toward H3K9me2when binding H3K4me3. Subunit: Interacts with POLR1B, UBTF, SETD1A, HCFC1, E2F1 andZNF711. Subcellular Location: Nucleus. Nucleus, nucleolus. Note=Recruitedto H3K4me3 sites on chromatin during interphase. Dissociates fromchromatin when cells enter mitosis. Post-translational modifications: Phosphorylation at Ser-69 and Ser-120 are required fordissociation from chromatin and accumulation of H4K20Me1 levelsduring prophase. DISEASE: Defects in PHF8 are the cause of mental retardationsyndromic X-linked Siderius type (MRXSSD) [MIM:300263]. A disordercharacterized by mild to borderline mental retardation with orwithout cleft lip/cleft palate. Similarity: Belongs to the JHDM1 histone demethylase family.JHDM1D subfamily. Contains 1 JmjC domain. Contains 1 PHD-type zinc finger. SWISS: Q9UPP1 Gene ID: 23133 Database links: Entrez Gene: 23133 Human Entrez Gene: 320595 Mouse SwissProt: Q9UPP1 Human SwissProt: Q80TJ7 Mouse |
| 產(chǎn)品圖片 |
Blank control(blue): Hela(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-PHF8/FITC Conjugated antibody (bs-8176R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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