| 產(chǎn)品編號(hào) | bs-8034R |
| 英文名稱(chēng) | COMMD1 Rabbit pAb |
| 中文名稱(chēng) | 銅代謝結(jié)構(gòu)域蛋白1抗體 |
| 別 名 | C2orf5; COMD1; COMD1_HUMAN; COMM domain-containing protein 1; COMMD1; copper metabolism domain containing 1; MGC27155; MURR1; Protein Murr1. |
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Specific References (1) | bs-8034R has been referenced in 1 publications.
[IF=3.541] Lili Zhang. et al. Disruption of COMMD1 accelerates diabetic atherosclerosis by promoting glycolysis. DIABETES VASC DIS RE. ;(): WB ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human COMMD1/MURR1: 95-190/190 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes. Tissue specificity: Ubiquitous. Highest expression in the liver, with lower expression in brain, lung, placenta, pancreas, small intestine, heart, skeletal muscle, kidney and placenta. Function: Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes. Subunit: Monomer and homodimer. Interacts (via COMM domain) with COMMD2, COMMD3, COMMD4, COMMD5, COMMD6, COMMD7, COMMD8 and COMMD10 (via COMM domain). Identified in a complex with an E3 ubiquitin ligase complex composed of TCEB1/elongin C, CUL2, SOCS1 and RBX1. Interacts directly with SOCS1 and CUL2. Interacts directly the N-terminal region of ATP7B. Interacts with CCS, CDKN2A, RELA and NFKBIB. Identified in a complex with NF-kappa-B. Interacts with CLU. Tissue Specificity: Ubiquitous. Highest expression in the liver, with lower expression in brain, lung, placenta, pancreas, small intestine, heart, skeletal muscle, kidney and placenta. Post-translational modifications: Ubiquitinated; undergoes both 'Lys-63'- and 'Lys-48'-linked polyubiquitination. Ubiquitinated by XIAP, leading to its proteasomal degradation. Similarity: Contains 1 COMM domain. SWISS: Q8N668 Gene ID: 150684 Database links: Entrez Gene: 150684 Human Entrez Gene: 17846 Mouse Omim: 607238 Human SwissProt: Q8N668 Human SwissProt: Q8K4M5 Mouse Unigene: 468702 Human Unigene: 259903 Mouse Unigene: 451238 Mouse COMMD蛋白家族中最典型的成員.它是一個(gè)多效性因子,參與許多生理活動(dòng),包括銅代謝、鈉運(yùn)輸、對(duì)核因子κB(NF-κB)及低氧誘導(dǎo)因子1(HIF-1)的調(diào)節(jié)等，COMMD廣泛存在于多細(xì)胞生物中,最典型的特征是它們的羧基端存在一個(gè)高度保守而獨(dú)特的結(jié)構(gòu)—COMMD結(jié)構(gòu)域,為蛋白質(zhì)間的相互作用提供了關(guān)鍵界面. 目前研究COMMD蛋白家族有10個(gè)成員,即COMMD1～10.COMMD1是COMMD蛋白家族中最先被證實(shí)且研究最為深入的蛋白質(zhì),在不同物種中廣泛表達(dá),在人類(lèi)的不同組織表達(dá)存在差異. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COMMD1) Polyclonal Antibody, Unconjugated (bs-8034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
4% Paraformaldehyde-fixed HepG2(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (COMMD1) polyclonal Antibody, unconjugated (bs-8034R) 1:50, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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