| 產(chǎn)品編號 | bs-2894R |
| 英文名稱 | HDAC11 Rabbit pAb |
| 中文名稱 | 組蛋白去乙酰化酶11抗體 |
| 別 名 | HD 11; HD11; HDAC 11; Histone deacetylase 11; FLJ22237; HDA11_HUMAN. |
| 研究領域 | 腫瘤 免疫學 信號轉(zhuǎn)導 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Chicken,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HDAC11: 21-120/347 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Histone deacetylases (HDAC) are a family of 11 enzymes that are involved in the regulation of gene activation and silencing by regulating chromatin structure. HDAC11 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Deacetylation of histones is associated with the condensation of chromatin to a compact structure (heterochromatin) in which the genes are silenced. Each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo. HDACs are known to be associated with a number of well characterized cellular oncogenes and tumour- suppressor genes and inhibitors of HDACs induce growth arrest, differentiation or apoptosis of cancer cells in vitro and in vivo. Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Subunit: Interacts with HDAC6. Subcellular Location: Nucleus. Note=Predominantly nuclear. Tissue Specificity: Weakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis. Similarity: Belongs to the histone deacetylase family. SWISS: Q96DB2 Gene ID: 79885 Database links: Entrez Gene: 79885 Human Entrez Gene: 232232 Mouse Omim: 607226 Human SwissProt: Q96DB2 Human SwissProt: Q91WA3 Mouse Unigene: 728246 Human Unigene: 744132 Human Unigene: 206218 Mouse Unigene: 28065 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HDAC11 Polyclonal Antibody, Unconjugated(bs-2894R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:HepG2.
Primary Antibody (green line): Rabbit Anti-HDAC11 antibody (bs-2894R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
