| 產(chǎn)品編號 | bsm-62758R |
| 英文名稱 | Phospho-c Jun/JunD (Ser73/Ser100) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化原癌基因c-Jun/JunD重組兔單抗 |
| 別 名 | JunD; c-Jun. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 15D9 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36,35 kDa |
| 檢測分子量 | 38,42,45 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human c Jun around the phosphorylation site of S73: LA-pS-PE |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity. SWISS: P05412 Gene ID: 3725 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators） C-jun(Oncoprotein C-jun：active protein 1)基因與鳥類肉瘤病毒17的轉(zhuǎn)化基因具有同源性，是早期應(yīng)答基因家族成員之一。主要用于各種惡性腫瘤的研究。C-jun又稱應(yīng)激活化蛋白激酶. |
| 產(chǎn)品圖片 |
Western blot analysis of RAW264.7 cell lysate. Using Phospho-c-Jun (Thr91+Thr93) (bsm-62758R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
