| 產(chǎn)品編號 | bsm-61230R |
| 英文名稱 | HTRA2 Recombinant Rabbit mAb |
| 中文名稱 | 線粒體絲氨酸蛋白酶重組兔單抗 |
| 別 名 | HTRA2; HtrA-like serine protease; OMI; PARK13; Protease; PRSS25; Serine protease 25; HtrA serine peptidase 2; Serine proteinase OMI. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | 38 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human HTRA2: 112-170/458 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. SWISS: O43464 Gene ID: 27429 HtrA2/Omi是一種寡聚絲氨酸蛋白酶,存在于大部分的正常組織器官中,當細胞受到凋亡刺激時,HtrA2/Omi可由線粒體膜間隙(mitochondrial intermembrane space)釋放進入細胞質(zhì)中,導(dǎo)致出現(xiàn)大量的細胞凋亡性死亡，Omi/HtrA2 為促凋亡因子。 |
| 產(chǎn)品圖片 |
Western blot analysis of Jurkat cell lysate. Using HTRA2 (bsm-61230R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
