| 產(chǎn)品編號 |
BSKM66332 |
| 英文名稱 |
Mouse Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit
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| 中文名稱 |
小鼠無翅型MMTV整合位點家族成員5A(WNT5A) ELISA Kit |
| 克 隆 號 |
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| 適用樣品基質(zhì) |
tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| 保存條件 |
Srore at 4℃ & -20℃ for 12 months. |
| 注意事項 |
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to WNT5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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